Method for increasing yield of cytoplasmic polyhedrosis virus of pine caterpillars

The invention relates to the technical field of proliferation of cytoplasmic polyhedrosis viruses of pine caterpillars, in particular to a method for increasing the yield of cytoplasmic polyhedrosis viruses of pine caterpillars. According to the method, artificial feed for replacing hosts is prepared from 8-12 parts of wheat germs, 8-12 parts of bean flour, 2-5 parts of yeast powder, 0.5-1 part of ascorbic acid, 0.05-0.1 part of multivitamins, 0.1-0.5 part of a preservative, 2-4 parts of carrageenan powder and 0-0.05 part of juvenile hormone analogues. The culture temperature of the alternative hosts is 26 +/-2 DEG C, the photoperiod is 14L: 10D, and the relative humidity is 60 +/-2%; the inoculation amount of the polyhedrosis virus is 2-7 [mu] L/head, the concentration of the polyhedrosis virus aqueous solution is 1 * 10 < 6 > PIB/mL to 5 * 10 < 6 > PIB/mL, and the total yield of the virus is increased by 98.3-239.2% through the technical scheme. 本申请涉及马尾松毛虫质型多角体病毒增殖的技术领域，尤其是涉及一种提高马尾松毛虫质型多角体病毒产量的方法。该方法中替代寄主人工