Mycobacterium tuberculosis c-di-AMP (adenosine monophosphate) clastic enzyme monoclonal antibody as well as preparation method and application thereof

The invention provides a mycobacterium tuberculosis c-di-AMP clastic enzyme monoclonal antibody and a preparation method and application thereof, and belongs to the technical field of biological medicine, amino acid sequences of a heavy chain variable region and a light chain variable region of the monoclonal antibody are respectively shown as SEQ ID No.1 and SEQ ID No.2; and nucleotide sequences for coding the heavy chain variable region and the light chain variable region of the monoclonal antibody are respectively shown as SEQ ID No.3 and SEQ ID No.4. The monoclonal antibody mAb provided by the invention can be used for specifically recognizing a natural mycobacterium tuberculosis c-di-AMP clastic enzyme CnpB; through analysis, the CDR sequence of the monoclonal antibody mAb is further determined, and a material is provided for laboratory CnpB and cyclic nucleotide molecule related research and clinical preparation of reagents for diagnosing mycobacterium tuberculosis infection and drugs or vaccines for tr