Preparation method and application of magnetic beads for nucleic acid extraction

The invention discloses a preparation method and application of magnetic beads for nucleic acid extraction. The preparation method of the magnetic beads for nucleic acid extraction comprises the following specific steps: firstly, dissolving metal iron salt in a mixed solution composed of water and a...

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Hauptverfasser: XU QUANGUI, WANG PEIHAO, CHEN JIANGNENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method and application of magnetic beads for nucleic acid extraction. The preparation method of the magnetic beads for nucleic acid extraction comprises the following specific steps: firstly, dissolving metal iron salt in a mixed solution composed of water and an alcohol solvent to prepare a metal iron salt solution; adding a sodium salt, a reducing agent and a complexing agent into the metal ferric salt solution, and uniformly stirring to obtain a mixed solution; adding the prepared mixed solution into a reaction kettle for hydrothermal reaction, washing the obtained solid with water and an alcohol solvent, and drying to obtain ferroferric oxide powder; the preparation method comprises the following steps: suspending ferroferric oxide powder in a mixed solution composed of water and an alcohol solvent, then adding a surfactant, estersil and alkali, heating the obtained mixed solution for reaction, washing the obtained solid with the water and the alcohol solvent, and dry