Pseudo-ginseng aseptic seedling cultivation method

The invention discloses a pseudo-ginseng aseptic seedling cultivation method which comprises the following steps: disinfection treatment: taking pseudo-ginseng seeds, disinfecting the pseudo-ginseng seeds with 75% alcohol for 1-3 minutes, disinfecting the pseudo-ginseng seeds with 13-17% sodium hypo...

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Bibliographische Detailangaben
Hauptverfasser: JIANG SHAN, YAO RUI, YAN LI, LU FAN, SUN YONGCAN, WU TIANJING, ZHANG LIYU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a pseudo-ginseng aseptic seedling cultivation method which comprises the following steps: disinfection treatment: taking pseudo-ginseng seeds, disinfecting the pseudo-ginseng seeds with 75% alcohol for 1-3 minutes, disinfecting the pseudo-ginseng seeds with 13-17% sodium hypochlorite for 10-21 minutes, and washing the pseudo-ginseng seeds with sterile water to obtain disinfected seeds; pollution screening: placing the disinfected seeds in a culture medium, observing for 7-12 days, and screening to obtain pollution-free seeds; and culturing: transferring pollution-free seeds into a tissue culture bottle, culturing for 12-16 days, taking out, transferring into the culture medium, continuously culturing for 1-12 days, observing pollution conditions, screening pollution-free plants, and transferring into the tissue culture bottle again to culture, thereby obtaining the aseptic pseudo-ginseng seedlings. The panax notoginseng aseptic seedling cultivation method is simple and non-toxic, the c