Colloidal gold immunochromatography rapid test strip for insect-resistant protein Cry 1Ah and detection method thereof

The invention discloses an insect-resistant protein Cry 1Ah colloidal gold immunochromatography rapid test strip and a detection method thereof. The test strip comprises a bottom plate as well as a sample pad, a gold mark pad, a reaction film and a water absorption pad which are adhered to the botto...

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Hauptverfasser: DONG MEI, GAO JIN, ZHAO WEILING, WANG RUIZHE, WANG ZHANCHAO, YANG XIUYING, LIU, WEIXIAO, WANG DI, WEN TINGTING, MENG LIXIA, WAN YUSONG, MIAO CHAOHUA, JIN WUJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses an insect-resistant protein Cry 1Ah colloidal gold immunochromatography rapid test strip and a detection method thereof. The test strip comprises a bottom plate as well as a sample pad, a gold mark pad, a reaction film and a water absorption pad which are adhered to the bottom plate, arranged from one end of the bottom plate to the other end of the bottom plate and sequentially connected, and is characterized in that the gold mark pad is coated with an insect-resistant protein Cry 1Ah monoclonal antibody-colloidal gold conjugate; the monoclonal antibody of the insect-resistant protein Cry 1Ah in the conjugate is obtained by secreting a hybridoma cell strain 3C4 2E6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.45483. The test strip disclosed by the invention is strong in specificity, high in sensitivity, simple, convenient, rapid and accurate to operate, and suitable for on-site rapid detection and safety evaluation of the insect-res