Method for producing itaconic acid through compound mutagenesis aspergillus terreus fermentation

The invention belongs to the technical field of microbial mutagenesis, and particularly discloses a method for producing itaconic acid through compound mutagenesis aspergillus terreus fermentation. The method is used for producing itaconic acid through mutagenesis aspergillus terreus fermentation. T...

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Hauptverfasser: WANG LINGLING, HAN PING, YIN SHAOCHUN, WANG ZHENYU, XU BINGZHENG, ZHENG XUEQI, ZHANG JIAN, LI YUEMING, MA HONGMEI, LI XIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of microbial mutagenesis, and particularly discloses a method for producing itaconic acid through compound mutagenesis aspergillus terreus fermentation. The method is used for producing itaconic acid through mutagenesis aspergillus terreus fermentation. The method comprises the following steps: activating an aspergillus terreus strain to prepare a spore suspension; the spore suspension is placed in a sterile culture dish to be subjected to ultraviolet mutagenesis, the ultraviolet mutagenesis time is 60-240 s, and the irradiation distance of an ultraviolet irradiation lamp is 30 cm; then, uniformly coating a flat plate with the spore suspension, and culturing for 3 days at 32 DEG C under the action of a static magnetic field in a dark environment, with the intensity of the static magnetic field being 10-50mT; after culture is finished, picking single colonies, inoculating the single colonies to a test tube inclined plane, and culturing at 32 DEG C for 6 days to obta