Preparation method of recombinant ascorbic acid oxidase

The invention discloses a preparation method of recombinant ascorbic acid oxidase. The preparation method of ascorbic acid oxidase provided by the invention depends on pichia pastoris as a heterologous expression fungus host system, takes methanol as a unique carbon source, and has the advantages of stable inheritance, low cost, easy culture, large scale, post-translational modification, secretory expression and the like; and the ascorbic acid oxidase protein with low cost and high enzyme activity can be produced on a large scale. 本申请公开了一种重组抗坏血酸氧化酶的制备方法。本申请中提供的抗坏血酸氧化酶的制备方法，依赖毕赤酵母作为异源表达的真菌宿主系统，以甲醇为唯一碳源，具有可稳定遗传、成本低易培养、可规模化、可翻译后修饰以及分泌表达等优势；可获得低成本、大规模生产高酶活的抗坏血酸氧化酶蛋白。