Kit for detecting and identifying swine fever, African swine fever and swine vesicular disease and application thereof

The invention belongs to the technical field of gene detection, and discloses a kit for detecting and identifying swine fever, African swine fever and swine vesicular disease and application, the kit comprises a primer and a probe group for detecting classical swine fever virus; the primer and the p...

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Hauptverfasser: DENG CONGLIANG, ZHANG XIAOHAN, WANG LIN, GAO ZHIQIANG, REN TONG, PU JING, JING SHANNA, XU ZIHAN, DU SILE, LI ZHUO, ZHAO XIANGPENG, LAI PING'AN, BAI ZILONG, LIU QUANGUO, SHI XIJU, ZHONG YAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene detection, and discloses a kit for detecting and identifying swine fever, African swine fever and swine vesicular disease and application, the kit comprises a primer and a probe group for detecting classical swine fever virus; the primer and the probe group are used for detecting the African swine fever virus; the primer and the probe set are used for detecting the swine vesicular disease virus; a chemical modification reagent; a viral nucleic acid extraction reagent; an RT reaction reagent; performing negative control; positive control; high-throughput sequencing test paper; a fluorescent RT-PCR reaction solution; and specification. The kit provided by the invention has multiple properties: the classical swine fever virus, the African swine fever virus and the swine vesicular disease virus can be simultaneously detected and distinguished, the detection time and the detection cost are saved, timely definite diagnosis of epidemic diseases, especially mixed i