Simoa kit for detecting content of biomarker hsa-miR-320b and application

The invention relates to the technical field of immunodetection, in particular to a Simoa kit for detecting the content of a biomarker hsa-miR-320b and application of the Simoa kit. The Simoa kit comprises an hsa-miR-320b capture probe, an hsa-miR-320b detection probe, a magnetic bead coated with the hsa-miR-320b capture probe, an hsa-miR-320b standard substance, an S [beta] G solution, a fluorescent substrate solution and a sample diluent. The lowest detection limit of the Simoa kit disclosed by the invention reaches 0.462 fM, the detection range is 1-10000 fM, and the content of hsa-miR-320b in human serum can be accurately detected. 本申请涉及免疫检测技术领域，尤其是一种检测生物标志物hsa-miR-320b含量的Simoa试剂盒和应用；所述Simoa试剂盒包括hsa-miR-320b捕获探针、hsa-miR-320b检测探针、hsa-miR-320b捕获探针包被的磁珠、hsa-miR-320b标准品、SβG溶液、荧光底物溶液和样本稀释液。本申请的Simoa试剂盒的最低检测限达到0.462fM，检测范围为1-10000fM，能够准确检测人血清中hsa-miR-320b含量。