Application method of composition for detecting various respiratory viruses and kit

The invention belongs to the technical field of gene detection, and discloses an application method of a composition for detecting various respiratory viruses and a kit. Monitoring an interior label; preparing a kit: preparing the kit for detecting various respiratory viruses according to the design...

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Bibliographische Detailangaben
Hauptverfasser: DENG CONGLIANG, WANG LIN, ZHANG XIAOHAN, GAO ZHIQIANG, REN TONG, PU JING, XU LIWEI, AN NING, DU SILE, BAI ZILONG, LAI PING'AN, LIU QUANGUO, ZHANG WEI, SHI XIJU, ZHONG YAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene detection, and discloses an application method of a composition for detecting various respiratory viruses and a kit. Monitoring an interior label; preparing a kit: preparing the kit for detecting various respiratory viruses according to the designed primer, probe and fluorescence reporter group; the kit should contain all necessary reagents; a respiratory tract sample of a patient is collected, viral nucleic acid in the respiratory tract sample is extracted, and then the provided kit is used for real-time fluorescence RT-PCR detection; analyzing the content of viruses in the sample; and a basis is provided for clinical diagnosis according to a detection result. According to the present invention, the respiratory tract infection viruses can be rapidly detected and identified, the six human respiratory tract infection viruses and the two porcine respiratory tract infection viruses possibly causing the human-livestock co-infection can be detected and identifie