SgRNA expression vector, CRISPR gene editing expression plasmid and preparation method of sgRNA expression vector and CRISPR gene editing expression plasmid

The invention relates to the technical field of biology, in particular to an sgRNA expression vector, a CRISPR (clustered regularly interspaced short palindromic repeats) gene editing expression plasmid and a preparation method of the sgRNA expression vector. The expression vector comprises an improved sgRNA expression module connected with a nucleotide sequence for expressing green fluorescent protein, wherein the nucleotide sequence of the improved sgRNA expression module comprises a nucleotide sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is greater than or equal to 90%; according to the present invention, the plasmid correctly connected with the sgRNA expression module and the target sequence can be rapidly screened by using whether the green fluorescent protein is expressed or not, such that the PCR verification does not need to be performed, and the sgRNA expression vector and the guide RNA plasmid do not need to be reconstructed each time since the target sequence