Lotus tissue culture propagation culture method

The invention discloses a curcuma alismatifolia tissue culture and propagation culture method. The method comprises the following steps: 1) preparing and disinfecting a plant; (2) inducing and culturing dormant buds; (3) subculture multiplication culture; 4) rooting and seedling strengthening culture; according to the culture method disclosed by the invention, a flower bud permeable culture medium, a subculture multiplication culture medium and a seedling strengthening and rooting culture medium are sequentially used in the culture process of each link, the temperature, humidity and illumination conditions are reasonably controlled, excellent variety seedlings of curcuma alismatifolia can be successfully cloned in batches within 95 days at the shortest, and then a large number of seedlings which are neat in plant type and high in yield can be obtained through cultivation. Therefore, the requirements of flower environment landscape are met. 本发明公开了一种姜荷花组培扩繁培养方法，包括如下步骤：1)植体制备与消毒；2)休眠芽诱导培养；3)继代增殖培养；4)生根壮苗培养；5)炼苗和