Method for establishing cardamine violifolia leaf tissue culture regeneration system

The invention discloses an establishment method of a cardamine violifolia leaf tissue culture regeneration system, a tissue culture medium for cardamine violifolia and application of the tissue culture medium. The method comprises the following steps: by taking cardamine violifolia leaves as explants, cleaning and sterilizing the leaves, inoculating the leaves into an induction culture medium, inducing for 15-20 days to obtain calluses, then transferring the calluses into a differential culture medium, culturing for 20-25 days, transferring buds with the height of 1-3 cm into a rooting culture medium, growing for 15-20 days, inducing rooting to form complete plants, and transplanting the complete plants. According to the cardamine violifolia leaf tissue culture regeneration system established by the method, cardamine violifolia explant regeneration seedlings can be efficiently obtained, a foundation can be laid for constructing a cardamine violifolia genetic transformation system, a technical reference is pro