Capture constructs and methods for detecting multiple analytes

A capture construct (100, 1000) for capturing a plurality of analytes (402, 504, 604, 706) of a biological sample (1300, 1408) is provided. The capture construct (100, 1000) comprises a nanostructured backbone (102), at least one first orientation indicator (104) and at least one second orientation indicator (106), and at least a plurality of first capture regions (108a-108f) on the nanostructured backbone (102), each capture region (108a-108f) comprising at least one affinity capture reagent (400, 502, 602, 702, 704), the affinity capture reagent (400, 502, 602, 702, 704) being configured to capture the analyte (402, 504, 604, 705). 706) of the plurality of analytes. In another aspect, a method of detecting a plurality of analytes (402, 504, 604, 706) of a biological sample (1300, 1408) is provided. 提供了一种用于捕获生物样品(1300,1408)的多个分析物(402,504,604,706)的捕获构建体(100,1000)。所述捕获构建体(100,1000)包含纳米结构骨架(102)、至少一个第一方位指示器(104)和至少一个第二方位指示器(106)、和在所述纳米结构骨架(102)上的至少多个第一捕获区域(108a-108f)，每个捕获区域(108a-108f)包含至少一种亲和捕获试剂(400,502,602,70