Preparation method of functional nanofiber co-loaded with probiotics

The invention provides a preparation method of functional nanofiber co-loaded with probiotics, which comprises the following steps: adding probiotics into a spinning solution, and preparing the functional nanofiber by using an electrostatic spinning technology. The spinning solution contains pullulan and a milk fat globule membrane, and the mass ratio of the pullulan to the milk fat globule membrane ranges from 80: 20 to 20: 80; the mass percent concentration of the pullulan and the milk fat globule membrane in the spinning solution is 20%. The functional nanofiber can successfully package the probiotics, not only provides a high survival rate of the probiotics, but also improves the tolerance, viability and storage stability of the probiotics for resisting gastrointestinal fluid and cholate, and has great application potential in functional food. 本发明提供一种共载益生菌功能性纳米纤维的制备方法，是在纺丝液中加入益生菌，在使用静电纺丝技术制成功能性纳米纤维。所述的纺丝液中包含有普鲁兰多糖和乳脂肪球膜，其质量比的范围为80：20至20：80；且纺丝液中普鲁兰多糖和乳脂肪球膜的质量百分比浓度为20％。本发明的功能性纳米纤维能够成功封装益生菌，不仅提供了益生菌的高存活率，还提