MS2 virus-like particle as well as preparation method and application thereof

The invention provides an MS2 virus-like particle as well as a preparation method and application thereof. Specifically, the virus-like particle is formed by coding polynucleotide, and the polynucleotide comprises a nucleotide sequence for coding coat protein of escherichia coli MS2 bacteriophage and a coding sequence of a virus to be carried. The virus-like particle disclosed by the invention is high in packaging efficiency, high in yield and purity, and capable of carrying nucleic acid of various viruses, so that the virus-like particle can be used for preparing positive reference products and/or quality inspection products in virus detection. 本发明提供了一种MS2病毒样颗粒及其制备方法和应用。具体地，本发明的病毒样颗粒由多核苷酸编码而成，所述多核苷酸包括编码大肠杆菌MS2噬菌体的外壳蛋白的核苷酸序列和待搭载病毒的编码序列。本发明的病毒样颗粒包装效率高，产量和纯度高，可搭载多种病毒的核酸，因此可用于制备病毒检测中的阳性参考品和/或质检品。