Application of Palbociclib in preparation of CHO cell serum-free medium

The invention relates to the technical field of serum-free culture media. The invention provides an application of Palbociclib in preparation of a CHO (Chinese Hamster Ovary) cell serum-free culture medium. The CHO cell serum-free culture medium is prepared by adding Palbociclib, and the final concentration of the Palbociclib in the CHO cell serum-free culture medium is 2-50 [mu] M. The CHO cell serum-free culture medium further comprises vitamin C which is used in cooperation with Palbociclib, the expression level of target protein in CHO cells can be better improved, the specific productivity of the cells is improved, growth of the CHO cells is promoted, the viability and yield of the cells are improved, and meanwhile the purpose of reducing the production cost is achieved. 本发明涉及无血清培养基技术领域。本发明提供了Palbociclib在制备CHO细胞无血清培养基中的应用。通过添加Palbociclib制备一种CHO细胞无血清培养基，所述Palbociclib在该CHO细胞无血清培养基中的终浓度为2-50μM。本发明CHO细胞无血清培养基中还包括维生素C，与Palbociclib搭配使用，能够更好的提高目的蛋白在CHO细胞中的表达水平，增加细胞特异性生产力，促进CHO细胞生长、提高细胞活率和产量，同时达到降低生产成本的目的。