Method for continuously extracting protein and polyphenol from walnut male flowers based on eutectic solvent

The invention belongs to the field of protein and polyphenol extraction, and particularly relates to a method for continuously extracting protein and polyphenol from walnut male flowers based on a deep-eutectic solvent. The deep-eutectic solvent is used for effectively extracting the walnut male flower protein, the deep-eutectic solvent is easy and convenient to prepare, low in cost, good in solubility, low in toxicity and environmentally friendly, and the extraction rate of the walnut male flower protein can reach 95% or above. The walnut male flower polyphenol contained in the walnut male flower filter residue doped with the walnut male flower protein extracting solution supernatant is effectively extracted by utilizing 50% ethanol, and the extraction rate of the walnut male flower polyphenol can reach 90%. A microbial fermentation wall breaking method is utilized, safety and high efficiency are achieved, the walnut male flower protein and polyphenol dissolution effect is good, the dissolution rate is high,