Latex immunoturbidimetric kit for NGAL (neutrophil gelatinase associated lipocalin) and preparation method

The invention relates to the technical field of biochemical immune in-vitro diagnostic reagents, in particular to a latex immunoturbidimetric kit for NGAL and a preparation method thereof.The latex immunoturbidimetric kit for the NGAL comprises a first reagent and a second reagent, and the first reagent is prepared from 50-100 mmol/L of phosphate buffer, 0.3% of sodium chloride, 0.1% of sodium azide, 1.0% of citric acid, 0.1% of Tween 20, 0.1% of ethylene diamine tetraacetic acid and 0.5% of sodium hydroxide; and 1-3% of polyethylene glycol 6000. The reagent II is prepared from 10 to 20 mmol/L of phosphate buffer solution, 0.3 percent of sodium azide, 1 to 5 percent of bovine serum albumin, 0.4 percent of citric acid, 0.1 percent of triton-100, 20 to 30 percent of glycerol and 8 to 12 percent of neutrophil gelatinase associated lipocalin antibody sensitization latex. The detection kit is low in cost, high in sensitivity, good in anti-interference performance, good in clinical correlation and good in stability