Endocannabinoid biomarker composition as well as detection method and application thereof

The invention relates to an endocannabinoid biomarker composition and a detection method, the biomarker composition comprises the following components: AEA, OEA, LEA, PEA or SEA, and the detection method comprises the following steps: (1) providing a sample comprising blood or tissue from a detection object; (2) pre-treating the sample to obtain a sample to be detected; and (3) carrying out separation and analysis detection on the to-be-detected sample by adopting an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry system to obtain the content of AEA, OEA, LEA, PEA or SEA in the sample. According to the detection method, derivatization treatment is not needed, the matrix effect is reduced, the detection accuracy is improved, trace detection in a biological sample can be achieved, the detection sensitivity is improved, qualitative and quantitative detection of various endocannabinoids can be achieved through one-time operation, the analysis time is short, the detection re