Preparation method of electrode for detecting vitamins in serum

The invention relates to the technical field of detection of vitamins in serum, in particular to a preparation method of an electrode for detecting vitamins in serum, which comprises the following steps: separating vitamin E combined with extremely low density lipoprotein in blood, and measuring the...

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Bibliographische Detailangaben
Hauptverfasser: WANG WEIJIN, TIAN SONGKUI, LIU CUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of detection of vitamins in serum, in particular to a preparation method of an electrode for detecting vitamins in serum, which comprises the following steps: separating vitamin E combined with extremely low density lipoprotein in blood, and measuring the vitamin E in a buffer solution in a free manner; the method comprises the following steps: firstly, preparing acid required by acidification: mixing the acid diluted by 5-50 times with water according to a ratio of 1: 1-9: 1; the method has the beneficial effects that the vitamin E combined with extremely low density lipoprotein in a human body is separated, and the vitamin E combined with the human body travels in a system, so that the real vitamin E in the human body is detected, and the detection accuracy is ensured. 本发明涉及血清中维生素检测技术领域,具体为一种用于血清中维生素检测的电极制备方法,包括:将血中与极低密度脂蛋白结合的维生素E分离开来并将其游离在缓冲液中被测量到,先配置酸化所需的酸:稀释5-50倍的酸与水以1:1-9:1的比例混合。有益效果为:本发明本发明使在人体里与极低密度脂蛋白结合的维生素E分离,并使人体里结合的维生素E游在体系中,保证测得的是真实的人体里的维生素E,确保检测的准确性。