Application of rice gene HL6 in regulation and control of diterpenoid phytoalexin content and enhancement of disease resistance

The invention discloses application of a rice gene HL6 in regulating and controlling the content of diterpenoid phytoalexin and enhancing disease resistance, the expression of HL6 protein is destroyed by knocking out the rice gene HL6 (the nucleotide sequence is as shown in SEQ ID NO.1), the expression level of diterpenoid phytoalexin synthetic genes (OsCPS2, OsKSL7, OsCPS4 and OsKSL4) in rice after the HL6 gene is knocked out is remarkably increased, and the disease resistance of the rice gene HL6 is enhanced. The content of diterpenoid phytoalexin such as rice husk ketone, rice leaf element and plant caraben is obviously increased, so that the disease resistance of the rice is improved. 本发明公开了水稻基因HL6在调控二萜植保素含量及增强抗病性中的应用，通过敲除水稻HL6基因（核苷酸序列如SEQ ID NO.1所示），破坏HL6蛋白表达，HL6基因敲除后水稻中二萜植保素合成基因（OsCPS2、OsKSL7、OsCPS4、OsKSL4）的表达水平显著升高，稻壳酮、稻叶素以及植物卡生等二萜植保素的含量显著增加，从而提高水稻抗病性。