Method for purifying mycobacterium nano-carrier protein
The invention provides a method for purifying mycobacterium nano-carrier protein, and belongs to the technical field of protein purification. The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli...
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| creator | DU ZHANQIANG GONG HONGRI |
| description | The invention provides a method for purifying mycobacterium nano-carrier protein, and belongs to the technical field of protein purification. The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli total protein solution contains mycobacterium nano-carrier protein, and a 1 * Flag tag is fused at the subunit C terminal of the mycobacterium nano-carrier protein; performing Flag tag affinity column chromatography purification on the escherichia coli total protein solution to obtain a mycobacterium nano-carrier protein crude extract; and carrying out gel exclusion column chromatography purification on the mycobacterium nano-carrier protein crude extract to obtain a pure mycobacterium nano-carrier protein product. The purification method provided by the invention is simple and easy to implement, and the obtained mycobacterium nano-carrier protein is large in yield and high in purity, and can be used for subsequent |
| format | Patent |
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The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli total protein solution contains mycobacterium nano-carrier protein, and a 1 * Flag tag is fused at the subunit C terminal of the mycobacterium nano-carrier protein; performing Flag tag affinity column chromatography purification on the escherichia coli total protein solution to obtain a mycobacterium nano-carrier protein crude extract; and carrying out gel exclusion column chromatography purification on the mycobacterium nano-carrier protein crude extract to obtain a pure mycobacterium nano-carrier protein product. The purification method provided by the invention is simple and easy to implement, and the obtained mycobacterium nano-carrier protein is large in yield and high in purity, and can be used for subsequent</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231117&DB=EPODOC&CC=CN&NR=117069862A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231117&DB=EPODOC&CC=CN&NR=117069862A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DU ZHANQIANG</creatorcontrib><creatorcontrib>GONG HONGRI</creatorcontrib><title>Method for purifying mycobacterium nano-carrier protein</title><description>The invention provides a method for purifying mycobacterium nano-carrier protein, and belongs to the technical field of protein purification. The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli total protein solution contains mycobacterium nano-carrier protein, and a 1 * Flag tag is fused at the subunit C terminal of the mycobacterium nano-carrier protein; performing Flag tag affinity column chromatography purification on the escherichia coli total protein solution to obtain a mycobacterium nano-carrier protein crude extract; and carrying out gel exclusion column chromatography purification on the mycobacterium nano-carrier protein crude extract to obtain a pure mycobacterium nano-carrier protein product. The purification method provided by the invention is simple and easy to implement, and the obtained mycobacterium nano-carrier protein is large in yield and high in purity, and can be used for subsequent</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD3TS3JyE9RSMsvUigoLcpMq8zMS1fIrUzOT0pMLkktyizNVchLzMvXTU4sKspMBSoqyi9JzczjYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GhuYGZpYWZkaOxsSoAQARRC6J</recordid><startdate>20231117</startdate><enddate>20231117</enddate><creator>DU ZHANQIANG</creator><creator>GONG HONGRI</creator><scope>EVB</scope></search><sort><creationdate>20231117</creationdate><title>Method for purifying mycobacterium nano-carrier protein</title><author>DU ZHANQIANG ; GONG HONGRI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117069862A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>DU ZHANQIANG</creatorcontrib><creatorcontrib>GONG HONGRI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DU ZHANQIANG</au><au>GONG HONGRI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for purifying mycobacterium nano-carrier protein</title><date>2023-11-17</date><risdate>2023</risdate><abstract>The invention provides a method for purifying mycobacterium nano-carrier protein, and belongs to the technical field of protein purification. The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli total protein solution contains mycobacterium nano-carrier protein, and a 1 * Flag tag is fused at the subunit C terminal of the mycobacterium nano-carrier protein; performing Flag tag affinity column chromatography purification on the escherichia coli total protein solution to obtain a mycobacterium nano-carrier protein crude extract; and carrying out gel exclusion column chromatography purification on the mycobacterium nano-carrier protein crude extract to obtain a pure mycobacterium nano-carrier protein product. The purification method provided by the invention is simple and easy to implement, and the obtained mycobacterium nano-carrier protein is large in yield and high in purity, and can be used for subsequent</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for purifying mycobacterium nano-carrier protein |
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