Method for purifying mycobacterium nano-carrier protein

The invention provides a method for purifying mycobacterium nano-carrier protein, and belongs to the technical field of protein purification. The purification method provided by the invention comprises the following steps: an escherichia coli total protein solution is provided, the escherichia coli total protein solution contains mycobacterium nano-carrier protein, and a 1 * Flag tag is fused at the subunit C terminal of the mycobacterium nano-carrier protein; performing Flag tag affinity column chromatography purification on the escherichia coli total protein solution to obtain a mycobacterium nano-carrier protein crude extract; and carrying out gel exclusion column chromatography purification on the mycobacterium nano-carrier protein crude extract to obtain a pure mycobacterium nano-carrier protein product. The purification method provided by the invention is simple and easy to implement, and the obtained mycobacterium nano-carrier protein is large in yield and high in purity, and can be used for subsequent