Trispecific and/or trivalent binding proteins for prevention or treatment of HIV infection

The present invention provides compositions comprising a trispecific and/or trivalent binding protein comprising four polypeptide chains forming three antigen binding sites that specifically bind to one or more HIV target proteins or one or more T cell receptors, wherein the first pair of polypeptides forming the binding protein has a dual variable domain with a cross-over orientation, and wherein the second pair of polypeptides forming the binding protein has a single variable domain. Also provided herein are methods for preparing trispecific and/or trivalent binding proteins and the use of such binding proteins for the treatment and/or prophylaxis of HIV/AIDS. 本发明提供包含三特异性和/或三价结合蛋白的组合物，所述三特异性和/或三价结合蛋白包含形成3个抗原结合位点的4条多肽链，所述3个抗原结合位点特异性结合一个或多个HIV靶蛋白或一个或多个T细胞受体，其中形成所述结合蛋白的第一对多肽具有双重可变域，其具有交叉(cross-over)取向，且其中形成所述结合蛋白的第二对多肽具有单一可变域。本文还提供用于制备三特异性和/或三价结合蛋白的方法和此类结合蛋白用于治疗和/或预防HIV/AIDS的用途。