Kit for detecting HPV IgG antibody and preparation method thereof

The invention relates to the technical field of detection, and particularly discloses a kit for detecting an HPV (human papillomavirus) IgG (immunoglobulin G) antibody and a preparation method of the kit. The kit comprises a mouse human IgG antibody-acridinium ester luminescent reagent and an HPV L1-VLP antigen-magnetic particle conjugate, the mouse source human IgG antibody-acridinium ester luminous reagent comprises a mouse source human IgG antibody and acridinium ester; the HPV L1-VLP antigen-magnetic particle conjugate comprises an HPV L1-VLP antigen and a magnetic particle, and the HPV L1-VLP antigen-magnetic particle conjugate comprises an HPV L1-VLP antigen and a magnetic particle; the HPV L1-VLP antigen is prepared by deleting an N-terminal sequence from an L1 gene of an HPV virus, modifying the L1 gene by point mutation, and performing protein expression, protein purification and particle assembly. The kit for detecting the HPV IgG antibody is high in sensitivity, strong in specificity and good in re