Thin-layer spectrum visual identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of thin-layer spectrum visual identification method

The invention belongs to the technical field of traditional Chinese medicine identification, and provides a thin-layer spectrum visualization identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of the thin-layer spectrum visualization identification method....

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Bibliographische Detailangaben
Hauptverfasser: XIE NINGJUN, XU SHAOJING, ZHANG QI, HUANG WENHUA, JIA YUWEI, YAN JIZHONG, YING XUHUI, ZHANG HUI, LIU LI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of traditional Chinese medicine identification, and provides a thin-layer spectrum visualization identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of the thin-layer spectrum visualization identification method.The thin-layer spectrum visualization identification method comprises the steps that a test sample is taken to be subjected to ultrasonic extraction, supernate is taken, and a test sample solution is obtained; carrying out sample application on the obtained test solution on a thin-layer plate to form a sample application original point, adding a developing agent, airing, spraying a color developing agent, heating, and recording a thin-layer chromatogram under ultraviolet light; converting the thin-layer chromatogram into a grey-scale map, and smoothing the grey-scale value information of the obtained grey-scale map to obtain a spectral grey-scale information curve of the test sample; performing hierarchical cluster