Thin-layer spectrum visual identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of thin-layer spectrum visual identification method
The invention belongs to the technical field of traditional Chinese medicine identification, and provides a thin-layer spectrum visualization identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of the thin-layer spectrum visualization identification method....
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the technical field of traditional Chinese medicine identification, and provides a thin-layer spectrum visualization identification method for pseudobulbus cremastrae seu pleiones and adulterants and application of the thin-layer spectrum visualization identification method.The thin-layer spectrum visualization identification method comprises the steps that a test sample is taken to be subjected to ultrasonic extraction, supernate is taken, and a test sample solution is obtained; carrying out sample application on the obtained test solution on a thin-layer plate to form a sample application original point, adding a developing agent, airing, spraying a color developing agent, heating, and recording a thin-layer chromatogram under ultraviolet light; converting the thin-layer chromatogram into a grey-scale map, and smoothing the grey-scale value information of the obtained grey-scale map to obtain a spectral grey-scale information curve of the test sample; performing hierarchical cluster |
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