Semi-quantitative measurement method for chemical components of different parts of single flue-cured tobacco leaf

The invention relates to a semi-quantitative measurement method for chemical components of different parts of a single flue-cured tobacco leaf, and belongs to the field of tobacco analytical chemistry research, and the method comprises the following steps: (1) selecting a plurality of target areas f...

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Hauptverfasser: PAN YANG, WANG WENBIN, HU YONGHUA, DIAO HONGLIN, LYU CHUNCHUN, SHAO NING, HE JINHUA, ZHANG JING, SHU JUNSHENG, LIU CHENGYUAN, HE ZENGYANG, NING YONG, JIANG YUTING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a semi-quantitative measurement method for chemical components of different parts of a single flue-cured tobacco leaf, and belongs to the field of tobacco analytical chemistry research, and the method comprises the following steps: (1) selecting a plurality of target areas from a petiole to a leaf tip on the tobacco leaf, and cutting out a tobacco leaf wafer in each target area; placing all the wafers on a polyfluortetraethylene plate, applying pressure by adopting a tablet press, leaving juice marks on the surface of the plate, and adhering the plate to a glass slide; (2) in a desorption electrospray and secondary photoionization mass spectrometry imaging device, analyzing a juice print by using an atomized solvent; the analytic compound is subjected to photoionization assisted by a dopant, and the ionic compound is subjected to mass spectrometric detection to obtain a mass spectrum; extracting spatial distribution information and molecular signal intensity of the detected compound i