Method for determining four tocopherols in cosmetic-grade vegetable oil

The invention relates to the technical field of cosmetic analysis, and provides a method for determining four tocopherols in cosmetic-grade vegetable oil in order to solve the problems that in the prior art, a method for detecting the tocopherols in the vegetable oil is poor in accuracy, tedious in detection steps, high in reagent toxicity and incapable of distinguishing alpha, beta, gamma and delta tocopherol isomers. Comprising the following steps: mixing vegetable oil to be detected with a protease solution, performing enzymolysis, adding a solvent, filtering to obtain filtrate, and detecting the filtrate by using reversed-phase liquid chromatography, the reversed-phase liquid chromatography conditions are as follows: a PFP column; the mobile phase A is water, and the mobile phase B is methanol; the flow velocity of the mobile phase is 0.8-1.2 mL/min; the column temperature is 30 DEG C, and gradient elution is carried out. The method is reliable, good in accuracy, simple in step and high in detection speed