Tibetan porcine parvovirus reverse transcription recombinase assisted amplification detection method

The invention discloses a Tibetan porcine parvovirus reverse transcription recombinase-assisted amplification detection method. The detection method comprises the following steps: designing and synthesizing primer probes; constructing and identifying a recombinant plasmid standard substance; screening a primer probe, and selecting an optimal primer; reaction conditions are optimized, and the optimal primer probe concentration and the optimal reaction temperature are selected; and testing sensitivity, repeatability and specificity. The method has the beneficial effects that the reverse transcription recombinase-mediated amplification detection technology is adopted, so that the reaction is rapid, the required time is short, the requirement on instruments is simple, expensive PCR instruments (only a handheld portable constant temperature device needs to be carried) are not needed, a temperature control instrument for thermal cycle reaction is not needed, the reaction time is greatly shortened, and the method is