Taqman fluorescent quantitative PCR primer, probe and kit for detecting TTR-rs28933979 gene hotspot mutation

The invention discloses a Taqman fluorescent quantitative PCR (Polymerase Chain Reaction) primer, a Taqman fluorescent quantitative PCR probe and a Taqman fluorescent quantitative PCR kit for detecting hot spot mutation of a TTR-rs28933979 gene. The upstream primer and the downstream primer are shown as SEQ ID No: 1 and SEQ ID No: 2, and the probes are shown as SEQ ID No: 3 and SEQ ID No: 4. According to the method, TTR-rs28933979 site mutation in human blood is analyzed through Taqman fluorescent quantitative PCR, the method is good in stability, high in sensitivity and high in specificity, the human FAP disease virulence gene TTR-rs28933979 hot spot mutation can be detected within 1.5 hours, the method has the advantages of being rapid, capable of saving trouble, convenient to operate and the like, the TTR-rs28933979 GA or AA genotype of a human FAP patient can be accurately detected by adopting the Taqman fluorescent quantitative PCR technology, and the method is suitable for large-scale popularization and