AcMNPV strain as well as acquisition method and application thereof

The invention relates to the technical field of insect prevention and control, and discloses an AcMNPV strain, an acquisition method and application thereof, and the acquisition method comprises the following steps: S1, using cotton bollworm or beet armyworm larvae as a host to proliferate an autogr...

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Bibliographische Detailangaben
Hauptverfasser:	JIN WENYI, ZHANG JIHONG, MENG QIAN, QIN QILIAN, ZHANG HUAN, LI XUAN, WANG HONGTUO, GUO LIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of insect prevention and control, and discloses an AcMNPV strain, an acquisition method and application thereof, and the acquisition method comprises the following steps: S1, using cotton bollworm or beet armyworm larvae as a host to proliferate an autographa californica nuclear polyhedrosis virus; s2, performing continuous passage of the autographa californica nuclear polyhedrosis virus in the larva of the spodoptera frugiperda; and S3, continuous passage is carried out to screen and obtain virus strains with higher fatality rate and higher lethal speed for spodoptera frugiperda. The AcMNPV strain (AcMNPV-Sf-P10 strain) has the characteristics of wide insecticidal spectrum and high toxicity, so that the insecticidal activity of the AcMNPV strain on the semi-accepted host spodoptera frugiperda is enhanced, and the AcMNPV strain shows higher toxicity and higher lethal speed. The AcMNPV-Sf-P10 strain has good adaptability and can be used as an excellent production st