Full-automatic plasmid production system and preparation method

According to the full-automatic plasmid production system and the preparation method disclosed by the invention, a bacterial suspension, an alkali lysis solution and a neutralization solution are subjected to flow type mixing through a static suspension device, thalli are continuously segmented and mixed, the efficiency of alkali lysis and neutralization is improved, and the yield of plasmids is further improved. When plasmid DNA is collected, a strategy of combining precipitation and floating is adopted, and the method is particularly suitable for large-scale and automatic plasmid production. According to the method, the DNA precipitation efficiency can be improved, a middle clear liquid layer and upper layer suspended impurities are better layered, the turbidity of the middle layer clear liquid is remarkably reduced, and subsequent separation and filtration are facilitated. No matter before filtering or after filtering, the DNA yield higher than that of a traditional method is obtained. 本发明公开了一种质粒生产全自动系统及制备