Antibody for specifically detecting IL-7 and application

The invention relates to the technical fields of cellular immunology, molecular biology and antibody preparation, in particular to an antibody for specifically detecting IL-7 and application. The antibody for specifically detecting the IL-7 has a special spatial epitope, and shows high affinity and...

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Bibliographische Detailangaben
Hauptverfasser: MIAO JINGYUN, CHEN YIDING, DU CHENGWEI, WANG HENGLING, GE PINGJU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical fields of cellular immunology, molecular biology and antibody preparation, in particular to an antibody for specifically detecting IL-7 and application. The antibody for specifically detecting the IL-7 has a special spatial epitope, and shows high affinity and high specificity to the IL-7. The two antibodies provided by the invention are jointly used for an IL-7 paired antibody, the content of IL-7 in a culture medium and serum can be specifically detected through a sandwich method, the affinity is high, other cell factors are not combined, and the antibody combination is an ideal antibody combination for detecting the content of IL-7 protein and can be used for detecting IL-7 residues in a process for researching or producing T cells, NK cells and CAR-T cells. And the research and development process of the CGT therapy can be accelerated. 本发明涉及细胞免疫学、分子生物学和抗体制备技术领域,尤其涉及特异性检测IL-7的抗体及应用。本发明所述特异性检测IL-7的抗体具有特殊的空间表位,对IL-7表现出高亲和力及高特异性。将本发明提供的两种抗体共同用于IL-7的配对抗体,通过夹心法可特异性检测培养基以及血