Establishment method and application of lepidoptera insect sterile system

The invention relates to the technical field of biology, in particular to an establishment method of a lepidoptera insect sterile system and application of the lepidoptera insect sterile system in microbial function research. The establishment method of the lepidoptera insect sterile system is as shown in Figure 1, and comprises the following steps: collecting, cleaning, disinfecting and treating insect eggs, and breeding sterile larvae after larva incubation. According to the establishment method of the sterile insect system, the lepidoptera insects which are completely sterile can be prepared, the method is used for large-scale breeding of the sterile insects, and the method is simple, convenient to operate, economical and practical. Besides, by means of the obtained sterile insect system, insects with limited microorganisms can be obtained by supplementing specific microorganisms back into the sterile insect system through a certain method, and therefore the effects of the limited microorganisms in growth