Method for determining 144 steroid hormones in environmental sample by liquid chromatography-mass spectrometry

The invention discloses a method for simultaneously determining 144 steroid hormones in an environmental sample through liquid chromatography-mass spectrometry. Mass spectrometry parameters of the 144 steroid hormones are established and optimized, optimal mass spectrometry conditions are selected, a chromatographic column is optimized, the composition and proportion of a mobile phase are determined, the flow velocity and the column temperature are optimized, and the detection sensitivity, the chromatographic peak and the chromatography-mass spectrometry are optimized. Compared with the prior art, the method provided by the invention has the advantages of wide coverage of substance types and high detection flux, can meet the detection of various steroid hormones in environmental samples, and ensures the effective recovery rate and repeatability of the samples to the greatest extent. 本发明公开了液相色谱质谱联用同时测定环境样品中144种类固醇激素的方法，建立优化了144种类固醇激素的质谱参数，选取最佳质谱条件，优化色谱柱，确定流动相组成及比例，优化流速及柱温，最优化检测灵敏度及色谱峰及色谱质谱。与现有方法相比，本发明物质种类覆盖面广，