Probe composition for EBER in-situ hybridization detection, kit and application thereof

The invention discloses a probe composition and a kit for EBER in-situ hybridization detection and application of the probe composition and the kit. The probe composition comprises a first probe with a nucleotide sequence as shown in SEQ ID No.1 and a second probe with a nucleotide sequence as shown in SEQ ID No.2, the 3'ends of the first probe and the second probe are marked by markers and can be hybridized with EBER1/EBER2 of a sample at the same time, so that the sensitivity and sensitivity of detection are greatly improved. Meanwhile, in the detection process, a Tris-EDTA buffer solution containing citraconic anhydride and dithiothreitol is used for carrying out high-temperature water bath treatment on a tissue sample, damage to a tissue structure in the treatment process is avoided, meanwhile, EBER in-situ hybridization detection and an immunohistochemical experiment can be carried out at the same time, and the efficiency of a pathological department is improved. 本发明公开了用于EBER原位杂交检测的探针组合物、试剂盒及其应用，所述探针组合物包