RPA-CRISPR/Cas12a primer group, gRNA, probe, kit and detection method

The invention belongs to the field of biology, and discloses an RPA-CRISPR/Cas12a primer group, gRNA and a probe for detecting haemophilus parasuis, and the RPA-CRISPR/Cas12a primer group comprises an RPA primer group, a gRNA and a probe; the RPA primer group comprises an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2; the sequence of the gRNA is as shown in SEQ ID NO. 3; the sequence of the probe is as shown in SEQ ID NO. 4; the end 5 and the end 3 of the probe are respectively a fluorophore and a quenching group. The method has the advantages of low detection limit and high detection accuracy. Meanwhile, the invention further discloses a kit and a detection method of the haemophilus parasuis. 本发明属于生物领域，公开了一种检测副猪嗜血杆菌的RPA-CRISPR/Cas12a引物组、gRNA和探针，包括RPA引物组、gRNA和探针；所述RPA引物组包括如SEQ ID NO.1和SEQ ID NO.2所述的上游引物和下游引物；gRNA的序列如SEQ ID NO.3所示；所述探针的序列如SEQ ID NO.4所示；所述探针的5端和3端分别为荧光基团和淬灭基团。其具有检出限低、检测准确性高的优点。同时，本发明还公开了一种试剂盒以及副猪嗜血杆菌的检测方法。