Anti-acid mycobacterium tuberculosis fluorescent staining kit as well as detection method and application thereof

The invention provides an anti-acid mycobacterium tuberculosis fluorescent staining kit and a detection method and application thereof, and belongs to the technical field of medicine and biochemistry. The anti-acid mycobacterium tuberculosis fluorescent staining kit comprises dewaxing liquid, staining liquid and mounting liquid which are independent from one another; the dewaxing liquid contains turpentine and tissue repair liquid which are independent of each other, and the tissue repair liquid is composed of triton 100 and ethylenediamine tetraacetic acid; the staining solution contains a rhodamine B solution, an auramine O solution and a hydrochloric acid alcohol solution which are independent from one another. The anti-acid tubercle bacillus fluorescent staining kit provided by the invention solves the problem of poor staining effect of the anti-acid tubercle bacillus in a paraffin tissue slice sample, and the detection rate of the anti-acid tubercle bacillus is relatively high. 本发明提供了一种抗酸结核杆菌荧光染色试剂盒及其检测方