Method and chip for capturing single circulating tumor cell and application of chip
The invention belongs to the technical field of biomedicine, and particularly relates to a method and a chip for capturing a single circulating tumor cell and application of the chip. The preparation method of the nano-porous graphene oxide chip comprises the following steps: placing graphene oxide...
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| creator | YAO WEIRONG GUI SHAOGAO ZENG LIN SHEN QINGLIN WANG YUNLONG FAN BING JIANG PIAO MAO ZHIFANG ZHOU ZHIYONG |
| description | The invention belongs to the technical field of biomedicine, and particularly relates to a method and a chip for capturing a single circulating tumor cell and application of the chip. The preparation method of the nano-porous graphene oxide chip comprises the following steps: placing graphene oxide in a chloroform solution of octadecyl ammonium chloride to form a graphene oxide/dioctadecyl dimethyl bromination solution which is marked as a GO/DODA solution, spin-coating the GO/DODA solution on the surface of a substrate to form a nano-porous graphene oxide substrate, and drying the nano-porous graphene oxide substrate to obtain the nano-porous graphene oxide chip. The preparation method comprises the following steps: sequentially carrying out silanization treatment and amidation treatment on a nano-porous graphene oxide substrate, then bonding streptavidin, and finally modifying an antibody to obtain the nano-porous graphene oxide chip. The nano-porous graphene oxide chip disclosed by the invention can captur |
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The preparation method of the nano-porous graphene oxide chip comprises the following steps: placing graphene oxide in a chloroform solution of octadecyl ammonium chloride to form a graphene oxide/dioctadecyl dimethyl bromination solution which is marked as a GO/DODA solution, spin-coating the GO/DODA solution on the surface of a substrate to form a nano-porous graphene oxide substrate, and drying the nano-porous graphene oxide substrate to obtain the nano-porous graphene oxide chip. The preparation method comprises the following steps: sequentially carrying out silanization treatment and amidation treatment on a nano-porous graphene oxide substrate, then bonding streptavidin, and finally modifying an antibody to obtain the nano-porous graphene oxide chip. 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| language | chi ; eng |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE |
| title | Method and chip for capturing single circulating tumor cell and application of chip |
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