High-strength silk protein physical hydrogel as well as preparation method and application thereof

The invention relates to high-strength silk protein physical hydrogel as well as a preparation method and application thereof. According to the method, natural silkworm cocoons serve as raw materials, a high-concentration silk protein solution is prepared through the processes of degumming, dissolving, desalting, concentrating and the like, and gluconic acid delta-lactone serves as a protein denaturant to prepare the silk protein hydrogel. Meanwhile, calcium chloride is added, so that a silk protein molecular chain is converted into beta-folding under a limited condition, a beta-folding crystal region which is small and uniform in size is formed as a physical crosslinking point, and finally the high-strength silk protein physical crosslinking hydrogel is obtained. Compared with the prior art, the preparation conditions are green and mild, the used raw materials are non-toxic and harmless, and the economic cost is low. The prepared silk protein hydrogel is good in biocompatibility, degradable and excellent in