Method for detecting content of heat-sensitive active protein in liquid milk

The invention provides a method for detecting the content of heat-sensitive active protein in liquid milk, which adopts ultra-high performance liquid chromatography for detection and comprises the following steps: 1) preparing a standard solution; 2) preparing a to-be-detected sample: adding water into the to-be-detected liquid milk sample for dilution, adjusting the pH value, precipitating casein, adding water for constant volume, and sequentially carrying out vortex oscillation, standing, low-temperature centrifugal treatment and filtering to obtain the to-be-detected sample; 3) sample detection: detecting the standard working solution and the sample to be detected by liquid chromatography, drawing a standard working curve, and establishing a standard equation; according to the standard curve and the standard equation, performing qualitative and quantitative detection on the active protein components in the sample to be detected; the conditions of the liquid chromatography are as follows: a chromatographic