Alginic acid oligosaccharide content determination method and application thereof

The invention discloses a method for determining the content of alginic acid oligosaccharide. The method comprises the following steps: completely converting C6-position carboxyl of alginic acid oligosaccharide into carboxyl salt, based on a charge conservation principle, calcining and ashing to change the carboxyl salt into carbonate, determining the amount of carbonate by an acid-base titration method, and converting according to monosaccharide residues to obtain the content of alginic acid oligosaccharide. According to the method, alginic acid oligosaccharide does not need to be degraded into monosaccharide, and the problems that the degradation degree is difficult to control and the degradation damage rate affects content determination are solved. According to the method, monosaccharide or oligosaccharide reference substances do not need to be used, different mannuronic acid/guluronic acid ratios do not affect the content measurement result, a standard curve does not need to be drawn, operation is easy an