FLOW CYTOMETER INCLUDING AN INCLINED BEAM SHAPING OPTICAL

A flow cytometer including a tilted beam shaping optic is provided. In certain embodiments, the subject flow cytometry includes a flow cell, a light source configured to generate a light beam at a probe point for irradiating particles in the flow cell, and a tilted beam shaping optic located between the light source and the flow cell. In such embodiments, the tilted beam shaping optical component is configured to generate beam ovality by creating astigmatism in the beam. In some embodiments, the tilted beam shaping optical component is a lens. In other embodiments, the oblique beam shaping optical component is a concave mirror. Also provided are methods of analyzing a sample using a flow cytometer including a tilted beam shaping optical component. 提供了包括倾斜光束整形光学部件的流式细胞仪。在某些实施方式中，主题流式细胞仪包括流动池、被配置成在探查点处产生用于辐照流动池中的粒子的光束的光源以及位于光源和流动池之间的倾斜光束整形光学部件。在这样的实施方式中，倾斜光束整形光学部件被配置成通过在光束中创建像散来生成光束椭圆度。在一些实施方式中，倾斜光束整形光学部件是透镜。在其他实施方式中，倾斜光束整形光学部件是凹面镜。还提供了使用包括倾斜光束整形光学部件的流式细胞仪分析样本的方法。