Screening and application of reference genes stably expressed in different developmental stages of Louyi armyworm

The invention discloses a screening method of reference genes stably expressed in different developmental stages of armyworm louyi and application of the reference genes. The reference genes are alpha-TUB and Actin; the nucleotide sequence of the reference gene alpha-TUB is shown as ID 1, and the nu...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG TAO, NING JUN, ZHANG GUOHANG, YANG CHAOXIA, YANG XINLING, WANG LIUYANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a screening method of reference genes stably expressed in different developmental stages of armyworm louyi and application of the reference genes. The reference genes are alpha-TUB and Actin; the nucleotide sequence of the reference gene alpha-TUB is shown as ID 1, and the nucleotide sequence of the reference gene Actin is shown as ID 2. According to a transcriptome analysis result obtained in a laboratory and reported reference gene information in other lepidoptera insects, nine stable candidate reference genes are screened out, and gene stability evaluation is carried out by utilizing a qRT-PCR technology, GeNorm, BestKeeper, a delta Ct method and NormFinder. And finally, performing comprehensive comparison and stability evaluation on all candidate reference genes through a RefFinder online website to obtain that alpha-TUB and Actin are reference genes stably expressed in different development stages of armyworm. According to the invention, a screening system for the stable reference