Preparation method of cladosporium fungus protoplast and construction method of genetic transformation system

The invention belongs to the technical field of microbial genetic transformation, and particularly relates to a preparation method of cladosporium fungus protoplast and a construction method of a genetic transformation system. According to the method, the enzymatic hydrolysate is prepared by dissolving the phosphate buffer solution, the cladosporium hyphae are split to prepare the protoplast, and the obtained protoplast is proper in quantity and good in quality. The Cladosporidium fungus protoplast obtained by the method can be used for effectively constructing and carrying out PEG (Polyethylene Glycol) mediated genetic transformation on the Cladosporidium fungus. 本发明属于微生物遗传转化技术领域，具体涉及一种枝孢属真菌原生质体的制备方法及遗传转化体系的构建方法。本发明以磷酸缓冲液溶解制备酶解液对枝孢属真菌菌丝进行裂解制备其原生质体，获得的原生质体数量适宜，质量好。以此方法获得的枝孢属(Cladosporium angulosum)真菌原生质体可以有效构建地进行PEG介导的枝孢属真菌遗传转化。