Quality control method, device and equipment for high-throughput sequencing data and storage medium

The invention relates to a quality control method and device for high-throughput sequencing data, equipment and a storage medium. The quality control method for the high-throughput sequencing data comprises the following steps: acquiring sample pollution information of each sample in an original sample set of one batch; in the process of screening whether abnormal samples exist in the original sample set or not, at least according to the sample pollution information, abnormal samples with the sample pollution information meeting a fourth preset condition are screened out from the original sample set, and an abnormal sample set is obtained; the fourth preset condition is determined according to a sample pollution degree preset range; and if the number of the abnormal samples in the abnormal sample set exceeds a number threshold value, generating and outputting an early warning prompt signal, and generating a data quality control report containing information of the abnormal samples in the abnormal sample set.