Nano polymerase labeled antibody as well as preparation method and application thereof

The invention provides a nano polymerase labeled antibody as well as a preparation method and application thereof, the nano polymerase labeled antibody comprises a dendritic high-molecular polymer, enzymes, chain macromolecular polysaccharide and an antibody, and the dendritic high-molecular polymer...

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Hauptverfasser: QIU SHIQI, NING YAOHUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a nano polymerase labeled antibody as well as a preparation method and application thereof, the nano polymerase labeled antibody comprises a dendritic high-molecular polymer, enzymes, chain macromolecular polysaccharide and an antibody, and the dendritic high-molecular polymer is loaded with a plurality of enzymes to form grape cluster type nano polymerase; the nano polymerase and the antibody are connected through the chain macromolecular polysaccharide to form a nano polymerase labeled antibody, the enzyme content is increased, the grape cluster type nano polymerase is formed, and the nano polymerase and the antibody are coupled with the chain macromolecular polysaccharide, so that the problems of compact space and large steric hindrance caused by direct binding to the antibody are solved; meanwhile, the enzyme content is also increased, so that the infiltration capacity of the nano polymerase labeled antibody is improved, and the detection sensitivity is improved. Reagents EDC/NHS an