Tag group for DNA library preparation, preparation method of tag group, DNA tag library and kit
The invention provides a tag group for DNA library preparation, a preparation method thereof, a DNA tag library and a kit. In label groups, each label group comprises x labels, and x is equal to 4n; the tag sequence in the tag group is a nucleotide sequence as shown in the formula 1, and (Nx) a = 5...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a tag group for DNA library preparation, a preparation method thereof, a DNA tag library and a kit. In label groups, each label group comprises x labels, and x is equal to 4n; the tag sequence in the tag group is a nucleotide sequence as shown in the formula 1, and (Nx) a = 5 '-Nx1Nx2Nx3... Nxa-3'; n is selected from any one of four base nucleotides of A, T, G and C, (N1) a-(Nx) a are all tag sequences in a group, each sequence is a nucleotide sequence composed of a bases, and a is a natural number greater than or equal to 8; in the tag group, in the sequences of N11-Nx1, N12-Nx2,..., N1a-Nxa in the group, A basic groups account for 25%, and G basic groups account for 25%; each tag sequence does not contain more than three continuous same basic groups; the last 8 bases of any two tag sequences are not mutually repeated. According to the label group for DNA library preparation, the reliability, usability and balance in the actual DNA library building process are improved, and the label g |
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