Norovirus nucleic acid detection method based on label-free ratio fluorescent PCR

The invention discloses a norovirus nucleic acid detection method based on label-free ratio fluorescent PCR (polymerase chain reaction), and belongs to the field of biology and detection. According to the invention, a G4 integrated PCR strategy of a GII type NoV genome is constructed, the characteristics of single excitation wavelength and double signal output of DAPI and NMM are utilized, a PCR product is detected by adopting a ratio fluorescence detection system, certain sensitivity and specificity are ensured while the cost is reduced, and a new thought is provided for detection of NoV and other food-borne pathogenic microorganisms. The method is based on a mature PCR technical system, simultaneous output of double fluorescence signals is realized through ingenious primer design, a ratio fluorescence PCR detection method is established, and detection of the GII type norovirus in food is expected to be realized. 本发明开发了一种基于免标记比率荧光PCR的诺如病毒核酸检测方法，属于生物和检测领域。本发明构建了一种GⅡ型NoV基因组的G4整合PCR策略，利用DAPI和NMM单激发波长、双信号输出的特点，采